Study note
Microsporum, epidermophyton, Trichophyton
Scytalidium hyalinum
Scytalidium has typical colony – fluffy white, If yes,
look for arthrospore
Arthrospore
Other Tinea should have - Aleurispore
A single terminal spore develops at the end of the conidiophores. It gets separated, and the conidiophore retains a scar.
No other spore develops there. The next spore develops from a branch below it. The detached spore has a large flat base.
Now we will look at these -
Colour of the colony – white, cream, brown, purple tinge
Consistency – granular, suede
Edge – glabrous
Back – brown, red, white, yellow, orange
Back- sharply defined or not sharply defined
Microscopy – Macroconidia mostly, or sparse
Macroconidia – smooth/rough surface, beak present/absent
Microconidia – shape – small, large, circular, ovoid, club-shaped,
Microconidia – broad base, narrow base
Hyphae – look for spiral hyphae
Conidia is a spore produced asexually by various fungi at the tip of a specialised hypha. If they are large, they are called macroconidia.
If it is present - is the outer surface rough? If yes, think Microsporum
M gypseum/fulvum
M canis/equinum –
if macroconidia large (>6 cell)= canis;
if small equinum
Ideally like this
Colony may look like this in young culture.
May not show typical feature
Trichophyton rubrum granular form. Look for macro and microconidia.
Microsporum persicolor
Trichophyton mentagrophytes
Trichophyton interdigitale
Urease +
Urease +, hair perforation test
(T rubrum – urease neg mostly)
T interdigitale,
T rubrum,
T interdigitale downy,
T rubrum granuler,
T tonsurans,
T erinacei
T soudanese,
T equinum,
Tinterdigitale noduler or
T erinacei
Fast growing. reverse dark brown
Fast growing. reverse dark orange
Fast growing, reverse bright yellow
Slow growing (>1 week)
Bright yellow back = T erinacei
Back red, cream or brown = Trichophyton interdigitale, T rubrum, tonsurans